Fig. 3: Inhibition of NK1R suppresses NSCLC cell progression in vitro. | Cell Death & Disease

Fig. 3: Inhibition of NK1R suppresses NSCLC cell progression in vitro.

From: Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR

Fig. 3

A Western blot analysis of NK1R expression in NSCLC cells transiently transfected with shRNA targeting NK1R or control shRNA. The effect of shRNA-mediated NK1R knockdown on B NSCLC cell colony formation (colonies larger than 0.1 mm in diameter were counted), C cell growth curve, D transwell cell migration, and E E-cadherin/vimentin, MMP2/14 protein expression. F Aprepitant inhibited the ability of cell colony formation stimulated by hHK-1. Aprepitant treatment induced NSCLC cell apoptosis measured by G flow cytometry analysis of PI/Annexin V co-staining and H western blot analysis of p53, Bax, Bcl-2, caspase3, cleaved-PARP protein level. I Band intensity of the p53, Bax, Bcl-2, caspase3, cleaved-PARP protein. migrated cell number, and densitometric analysis of each band were calculated with Image J software. Data were shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page