Fig. 5: The interaction between RSL1D1 and RAN is essential for autophagy and the proliferation and invasion of CRC cells.
From: RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression
A Co-IP combined with LC-MS/MS analysis was used to analyze the interacting proteins of RSL1D1 in CRC cells; RAN, a ras-related nuclear protein, was identified. B Co-IP of endogenous RSL1D1 and RAN proteins in CRC cells. C Double IF staining revealed the colocalization of RSL1D1 and RAN proteins in CRC cells. Scale bar, 10 µm. D The autophagic flux of CRC cells with stable RSL1D1 knockdown and transient RAN knockdown under starvation conditions was monitored by the mCherry-EGFP-LC3B assay. Scale bar, 50 µm. E Protein expression of LC3-II and P62 was examined by WB in CRC cells with stable RSL1D1 knockdown and transient RAN knockdown under starvation conditions. F–G. CCK-8 [F] and Transwell invasion assays [G] were used to detect the proliferation and invasion of CRC cells after stable RSL1D1 knockdown and transient RAN knockdown. Scale bar, 50 µm. *P < 0.05, **P < 0.01, ***P < 0.001, NS means no statistic difference. The error bars represent mean ± SD.
