Fig. 8: RSL1D1 inhibits the deacetylation of RAN and then promotes CRC cell proliferation and invasion by suppressing STAT3-regulated autophagy.
From: RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression
A IF analysis of STAT3 and p-STAT3 in CRC cells after RSL1D1 knockdown treated with NAM. Scale bar, 20 µm. B Total/nuclear/cytoplasmic extraction and western blotting detected the expression of STAT3 and p-STAT3Y705 in CRC cells after RSL1D1 knockdown and treatment with NAM. C Protein expression of LC3-II and P62 was examined by WB in CRC cells after RSL1D1 knockdown and treatment with NAM. D–E. CCK-8 [D] and Transwell invasion assays [E] were used to detect the proliferation and invasion of CRC cells after RSL1D1 knockdown and treatment with NAM. Scale bar, 100 µm. F Schematic representation of the function and potential mechanism of RSL1D1 in colorectal cancer. *P < 0.05, **P < 0.01, ***P < 0.001, NS means no statistic difference. The error bars represent mean ± SD.
