Fig. 5: ZC3H15 suppressed the transcription of E3 ligase CBL.
From: ZC3H15 promotes glioblastoma progression through regulating EGFR stability
A, B The protein level and mRNA level of several E3 ubiquitin ligases were detected in ZC3H15 knockdown and control cells. C The transcriptional activity of CBL in control and ZC3H15 knockdown and overexpression group were detected. D ChIP assay was performed by using Flag antibodies. IgG was used as the negative control. E The protein levels of ZC3H15, CBL, and EGFR were detected by western blot analysis. F, G The recovery of cellular proliferation was detected in ZC3H15-knockdown cells followed by CBL knockdown. H, I The recovery of the migration and invasion abilities in ZC3H15-knockdown cells followed by CBL knockdown. All data were expressed as mean ± SD. Student’s t test was performed to analyzed significance. *P < 0.05, **P < 0.01, ***P < 0.001.
