Fig. 2: POLE2 knockdown inhibits the viability and migration of GBM cells in vitro. | Cell Death & Disease

Fig. 2: POLE2 knockdown inhibits the viability and migration of GBM cells in vitro.

From: POLE2 facilitates the malignant phenotypes of glioblastoma through promoting AURKA-mediated stabilization of FOXM1

Fig. 2

A MTT cell viability assay was employed to show the effects of POLE2 on U87 and U251 cells. B Colony-forming ability of U87 and U251 cells was detected in shPOLE2 and shCtrl groups. C The U87 and U251 cell migration ability was accessed by Transwell assay. D The protein expression of N-cadherin, Vimentin, and snail of U87 and U251 cells was measured by WB. E The downstream protein expression of Akt, p-Akt, PIK3CA, CCND1, and CCNB1 of U251 cells was measured by WB. The representative images were selected from at least three independent experiments. Data were shown as mean ± SD. **P < 0.01, ***P < 0.001.

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