Fig. 3: POLE2 knockdown enhances apoptosis of GBM cells in vitro.

A Flow cytometry was performed to detect cell apoptosis of U87 and U251 cells with or without POLE2 knockdown. The representative images were selected from at least three independent experiments. B Human apoptosis antibody array was utilized to analyze the regulatory ability of POLE2 on expression of apoptosis-related proteins in U251 cells. C The protein levels of Bcl-2, HSP27/70, and Survivin in U87 and U251 cells with or without POLE2 knockdown were further analyzed by WB. Data were shown as mean ± SD. *P < 0.05, ***P < 0.001.