Fig. 1: Interaction between PRAS40 and PGK1. | Cell Death & Disease

Fig. 1: Interaction between PRAS40 and PGK1.

From: PGK1 represses autophagy-mediated cell death to promote the proliferation of liver cancer cells by phosphorylating PRAS40

Fig. 1

A–C Co-IP with anti-Myc antibody. The bands with red boxes were cut out and applied to mass spectrometry (A). The unique peptides of PGK1 enriched in Myc-PRAS40-bound precipitates were clarified by mass spectrometric analyses (B). Western Blotting analyses using the indicated antibodies (C). D Co-IP followed by western blotting analyses with anti-FLAG antibody. E GST pull-down assay using GST or GST-PRAS40 together with His-PGK1. F Immunofluorescent staining with anti-FLAG and anti-PRAS40 antibodies in the cells introduced with FLAG-PGK1. Scale bar, 10 μm. G Schematic diagram showing the deletion mutants of PRAS40. H, I Co-IP followed by western blotting analyses with the indicated antibodies in the indicated cells. The cells were treated with peptide control or peptide 241–256 at the indicated concentrations I.

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