Fig. 2: Correlation of PRAS40 phosphorylation with kinase PGK1. | Cell Death & Disease

Fig. 2: Correlation of PRAS40 phosphorylation with kinase PGK1.

From: PGK1 represses autophagy-mediated cell death to promote the proliferation of liver cancer cells by phosphorylating PRAS40

Fig. 2

A Sequencing result of a kinase-deficient mutant PGK1 T378P. The mutated nucleic acid was framed with a red box. B Coomassie blue staining image for the gel loaded with purified His-PGK1 or His-PGK1 T378P (left panel). Kinase assays using GST-PRAS40 together with His-PGK1 or His-PGK1 T378P (right panel) followed by western blotting analyses with the indicated antibodies. C–E Western blotting analyses with the indicated antibodies. The cells depleted with PGK1 were introduced with empty vector, shRNA-resistant FLAG-PGK1, or FLAG-PGK1 T378P expression vector (C, D). The indicated cells were treated with rapamycine (50 μM), API2 (100 μM), or LY294002 (50 μM) for 1 h (E). The quantification results of the band density were labeled above the bands.

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