Fig. 4: Tumor-infiltrating macrophages presented immunosuppressive in nccRCC TME. | Cell Death & Disease

Fig. 4: Tumor-infiltrating macrophages presented immunosuppressive in nccRCC TME.

From: Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study

Fig. 4

A tSNE plot of five subgroups of macrophages; B Violin plot of specific markers of each subgroup; C Heatmap of published marker of M2-poloarized like TAMs. Expression of each cluster was normalized into a row Z score; D Volcano plot of upregulated and downregulated genes between differently derived macrophages and normal macrophages; upregulated genes are colored in red, and downregulated genes are colored in blue. E Heatmap of already known immune checkpoints expression on macrophages. The row Z score represented the expression level; F Heatmap of expression regulated by transcription factors estimated by SCENIC among five clusters; red color represents active TFs; G, H The IHC analysis results of CD8+LAG3+ cells between para-tumor and tumor tissues. (random 20 fields and bar = 50 μm). I GSVA results of pathways enriched in each cluster of macrophages, and most cancer-associated pathways upregulated in tumor-infiltrating macrophages.

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