Fig. 3: PAARH silencing reduced HCC cellular malignant phenotype in vitro. | Cell Death & Disease

Fig. 3: PAARH silencing reduced HCC cellular malignant phenotype in vitro.

From: Long non-coding RNA PAARH promotes hepatocellular carcinoma progression and angiogenesis via upregulating HOTTIP and activating HIF-1α/VEGF signaling

Fig. 3

a PAARH expression in SNU-398 and Huh7 cells with PAARH stable silencing was measured by qRT-PCR. b Cell growth of SNU-398 and Huh7 cells with PAARH silencing was detected using CCK-8 assays. c Cell growth of SNU-398 and Huh7 cells with PAARH silencing was detected using EdU incorporation assays. Scale bars, 100 µm. d Cell apoptosis of SNU-398 and Huh7 cells with PAARH silencing was detected using caspase-3 activity assays. e Cell migration of SNU-398 and Huh7 cells with PAARH silencing was detected using transwell migration assays. Scale bars, 100 µm. f Cell invasion of SNU-398 and Huh7 cells with PAARH silencing was detected using transwell invasion assays. Scale bars, 100 µm. Results are shown as mean ± SD based on three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA followed by Dunnett’s multiple comparisons test.

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