Fig. 2: CRC cell-derived exosomal HSPC111 promotes CRLM in vivo.
A Representative images of mouse received intravenous injection of DiR-labeled HCT116 and SW480 cells-derived exosomes (left) and confocal microscopy of DiR-labeled exosomes in liver tissues (right). Scale bar = 20 μm. B The effects of ExoHCT116 and ExoSW480 on the activation of HSCs in mouse liver primary fibroblasts was analyzed by western blot. C Experimental scheme in exosomes and CRC cells induced CRLM mice model. D IVIS imaging on experimental liver metastasis of indicated mice treated with ExoHCT116 KD and their relative control. Luciferase-labeled HCT116 cells were used to perform experimental liver metastasis model (n = 6). E Representative pictures and quantitative results of hematoxylin and eosin (H&E) staining of liver tissue sections from indicated mice. Scale bar = 1 mm. F IVIS imaging on experimental liver metastasis of indicated mice treated with ExoSW480 OE and their relative control. Luciferase-labeled SW480 cells were used to perform experimental liver metastasis model (n = 6). G Representative pictures and quantitative results of HE staining of liver tissue sections from indicated mice. Scale bar = 1 mm. Each experiment was performed in triplicate. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.
