Fig. 2: HERC3 is validated to be downregulated in CRC and downregulated HERC3 predicts poor prognostic outcomes. | Cell Death & Disease

Fig. 2: HERC3 is validated to be downregulated in CRC and downregulated HERC3 predicts poor prognostic outcomes.

From: HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer

Fig. 2

HERC3 was indicated to be downregulated in CRC according to the results of qRT-PCR (a) and IHC (b) from the 70 paired CRC and adjacent-normal tissues. c Representative figures of the expression of HERC3 in paired CRC tumor tissues and adjacent-normal tissues based on the results of IHC, scale bars for low-magnification are 50 μm, for high-magnification are 25 μm. d Protein levels of HERC3 in 8 randomly selected paired CRC tissues and CRC adjacent-normal tissues detected by western blotting. e The mRNA expression level of HERC3 in NCM460, SW620, DLD-1, SW480, and HCT116 cell lines were detected by qRT-PCR. f The protein expression level of HERC3 in NCM460, SW620, DLD-1, SW480, and HCT116 cell lines were detected by western blotting. g OS and DFS analysis in 250 patients according to the expression of HERC3, 250 Patients were divided into two groups according to the median expression of HERC3. Survival analysis were visualized by Kaplan-Meier plots and tested by log-rank. P < 0.05 was identified as statistically significant.

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