Fig. 4: GAL1 overexpression does not alter the canalicular localization of P-gp in polarized HepG2 cells.

P-gp is visualized in HepG2, HepG2-M, and HepG2-GAL1 cells by immunostaining and epifluorescence microscopy (green fluorescence) and apical bile pseudocanaliculi structures are identified by dense F-actin stained with TRITC-phalloidin (red fluorescence). Nuclei were stained with DAPI (40X). Photographs are representative of three independent experiments.