Fig. 7: GAL-1 overexpression in PLC/PRF/5 HCC cells is not sufficient to protect cells from DOX-induced cell death. | Cell Death & Disease

Fig. 7: GAL-1 overexpression in PLC/PRF/5 HCC cells is not sufficient to protect cells from DOX-induced cell death.

From: Galectin-1 confers resistance to doxorubicin in hepatocellular carcinoma cells through modulation of P-glycoprotein expression

Fig. 7

A Western blot and densitometric analysis showing relative GAL1 expression in PLC/PRF/5 (PLC) and HepG2 cell lysates. β-tubulin was used as loading control (n = 9). *p < 0.05 with respect to HepG2 cells. B Western blot and densitometric analysis showing relative GAL1 expression in non-transfected PLC/PRF/5 (PLC) and transfected with pcDNA3.1-LGALS1 (PLC-GAL1) or expression vector without insert (PLC-M) cell lysates. β-tubulin was used as loading control (n = 7). ***p < 0.001 with respect to PLC-M cells. &&&p < 0.001 with respect to PLC cells. C Cell viability (MTT assay) in GAL1-overexpressing and control cells incubated with increasing concentrations of DOX (0–10 µM) for 48 h. Results are expressed as the mean of cell viability percentage with respect to the corresponding untreated cell line (100%) ± SEM (n = 10). The experimental data were fitted to dose–response curves by non-linear regression as described in “Materials and methods”. D Western blot showing P-gp expression in HepG2, PLC/PRF/5 (PLC), control PLC-M, and GAL1-overexpressing (PLC-GAL1) cell lysates. β-tubulin was used as loading control (n = 5).

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