Fig. 3: NCT increased mitochondrial mass in mouse liver human primary hepatocyte. | Cell Death & Disease

Fig. 3: NCT increased mitochondrial mass in mouse liver human primary hepatocyte.

From: Long-term oral administration of an HNF4α agonist prevents weight gain and hepatic steatosis by promoting increased mitochondrial mass and function

Fig. 3

A Liver sections from NC, HFD, and HFD + NCT mice were immunostained for VDAC-1 (red color). DAPI (blue) is for nuclear staining. B Quantification of hepatic VDAC-1 staining intensity (fold change vs. NC, NC, N = 3, HFD and HFD + NCT, N = 12). C Citrate synthase (CS) activity in liver lysate was reduced by HFD, which was reversed by NCT (NC, N = 3, HFD and HFD + NCT, N = 12). D Western blots for cytochrome C and SDHA. After detecting each protein, the membrane was stained for Ponceau S as a control for protein loading. E, F Quantification of cytochrome C and SDHA protein expression, respectively, in mouse liver. Each value was first normalized to the Ponceau S staining and the fold change was then calculated vs. NC (N = 6). G, H qPCR analysis in mouse liver for cytochrome C and SDHA mRNA expression normalized with 18s rRNA (NC, N = 3, HFD and HFD + NCT, N = 12). I, J qPCR analysis of cytochrome C and SDHA mRNA from human primary hepatocytes cultured in the indicated concentration of NCT (0, 5, 15, and 40 μM). Values were normalized with 18s rRNA (N = 3). K, L qPCR analysis in mouse liver for mitochondrial DNA (ND1, 16s) expression normalized with HK2 (N = 4). M qPCR analysis in mouse liver for HSP60 mRNA expression normalized with 18s rRNA (NC, N = 3, HFD and HFD + NCT, N = 12). N qPCR analysis in mouse liver for PPARγ mRNA expression normalized with 18s rRNA (NC, N = 3, HFD and HFD + NCT, N = 12). Each dot indicates an individual mouse or human donor. Values represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 (HFD vs. NC or HFD + NCT, 0 μM vs. each concentration of NCT in human hepatocyte). NS non-significant. Scale bar = 200 μM.

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