Fig. 2: DLEU2 regulated proliferation, migration, and invasion in prostate cancer cells. | Cell Death & Disease

Fig. 2: DLEU2 regulated proliferation, migration, and invasion in prostate cancer cells.

From: E2F transcription factor 2-activated DLEU2 contributes to prostate tumorigenesis by upregulating serum and glucocorticoid-induced protein kinase 1

Fig. 2

A qPCR analysis showing the overexpression and knockdown efficiency of DLEU2. B, C Analysis of cell viability in DLEU2 overexpression and knockdown prostate cancer cells using CCK-8 kit. D, E Result of EdU assay showing the cell proliferation in DLEU2 overexpression and knockdown prostate cancer cells (scale bar: 50 μm). F, G Colony formation assay was performed to determine the proliferation of PC-3 and DU145 cells harboring the different vectors indicated. H, I Transwell assays were performed to determine the migration and invasion capacity of PC-3 and DU145 cells transfecting with DLEU2 (scale bar: 50 μm). J, K Transwell assays were performed to determine the migration and invasion capacity of PC-3 and DU145 cells stably expressing shRNAs targeting DLEU2 (scale bar: 50μm). Data were indicated as mean ± SD, ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.

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