Fig. 3: BMP-7 inhibited high glucose-mediated Collagen-III expression and preserved cell phenotype of NRK-52E cell. | Cell Death & Disease

Fig. 3: BMP-7 inhibited high glucose-mediated Collagen-III expression and preserved cell phenotype of NRK-52E cell.

From: BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease

Fig. 3

Representative a Western blot and quantitative data were revealed the expression of b Collagen-III, c E-cadherin, in NRK-52E cells under normal-glucose condition, high glucose condition with either vehicle or recombinant human BMP-7(rhBMP-7) at different dosages,*P < 0.05 (n = 3). Representative d Western blot and quantitative data were revealed the expression of e Collagen-III, f E-cadherin, in NRK-52E cells under normal-glucose condition, high glucose condition with rhBMP-7(100 ng/ml) at different time, *P < 0.05 (n = 3). g Representative micrographs show immunofluorescence staining of E-cadherin in different groups. DAPI staining(blue) represents cell nucleus, Bar = 50 um. h Representative micrographs show immunofluorescence staining of α-SMA in different groups. DAPI staining(blue) represents cell nucleus, Bar = 50 um.

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