Fig. 4: BMP-7-restored SnoN protein countered renal fibrosis after diabetes. | Cell Death & Disease

Fig. 4: BMP-7-restored SnoN protein countered renal fibrosis after diabetes.

From: BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease

Fig. 4

a Graphical presentations show the relative abundance of SnoN mRNA after normalization with β-actin mRNA analyzed by RT-qPCR in the kidney tissues in different groups, *P < 0.05 (n = 6). b Western blotting and c quantitative data represent the expression of SnoN protein in the kidney tissues in different groups, *P < 0.05 (n = 6). d Graphical presentations show the relative abundance of SnoN mRNA after normalization with β-actin mRNA analyzed by RT-qPCR in NRK-52E cells under different conditions, *P < 0.05 (n = 3). e Western blotting and quantitative data represent the expression of SnoN protein in NRK-52E cells under different conditions, *P < 0.05 (n = 3). f Representative micrographs show immunofluorescence staining of SnoN in NRK-52E cells after incubation in different conditions. DAPI staining (blue) represents cell nucleus, Bar = 50 um. Knockdown of SnoN impaired the renoprotection of BMP-7 in NRK-52E cells under high-glucose conditions. g Western blotting and h quantitative data represent the expressions of E-cadherin, Collagen-III, and Vimentin in NRK-52E cells under different conditions, *P < 0.05 (n = 3).

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