Fig. 4: BMP-7-restored SnoN protein countered renal fibrosis after diabetes.

a Graphical presentations show the relative abundance of SnoN mRNA after normalization with β-actin mRNA analyzed by RT-qPCR in the kidney tissues in different groups, *P < 0.05 (n = 6). b Western blotting and c quantitative data represent the expression of SnoN protein in the kidney tissues in different groups, *P < 0.05 (n = 6). d Graphical presentations show the relative abundance of SnoN mRNA after normalization with β-actin mRNA analyzed by RT-qPCR in NRK-52E cells under different conditions, *P < 0.05 (n = 3). e Western blotting and quantitative data represent the expression of SnoN protein in NRK-52E cells under different conditions, *P < 0.05 (n = 3). f Representative micrographs show immunofluorescence staining of SnoN in NRK-52E cells after incubation in different conditions. DAPI staining (blue) represents cell nucleus, Bar = 50 um. Knockdown of SnoN impaired the renoprotection of BMP-7 in NRK-52E cells under high-glucose conditions. g Western blotting and h quantitative data represent the expressions of E-cadherin, Collagen-III, and Vimentin in NRK-52E cells under different conditions, *P < 0.05 (n = 3).