Fig. 6: Inhibition of Smad1/5 expression lessened BMP-7 induced SnoN protein and the renoprotection of BMP-7 in renal tubular interstitial fibrosis. | Cell Death & Disease

Fig. 6: Inhibition of Smad1/5 expression lessened BMP-7 induced SnoN protein and the renoprotection of BMP-7 in renal tubular interstitial fibrosis.

From: BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease

Fig. 6

Knockdown Smad1/5 by transfection of Smad1/5shRNA(sh-Samd1/5) plasmid. Representative a Western blot and quantitative data were revealed the expression of b p-Smad1/5(ser463/465), Smad1, Smad5, and d SnoN in NRK-52E cells under different conditions. c Graphical presentations show the relative abundance of SnoN mRNA after normalization with β-actin mRNA analyzed by RT-qPCR. Representative e Western blot and f quantitative data were revealed the expression of E-cadherin, Collagen-III, and Vimentin in NRK-52E cells under different conditions, *P < 0.05 (n = 3).

Back to article page