Fig. 8: BMP-7 modulates ubiquitination of SnoN in renal tubular cells during DN. | Cell Death & Disease

Fig. 8: BMP-7 modulates ubiquitination of SnoN in renal tubular cells during DN.

From: BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease

Fig. 8

Immunoprecipitation shows the phosphorylation and ubiquitination of SnoN in the kidney tissues and NRK-52E cells in different groups. a Input:whole renal tissue lysis from NC group NC + Ctr:vehicle injection in normal mice;NC + B:OE-BMP-7 plasmid injection in normal mice;DM + Ctr:vehicle injection in diabetic mice;DM + B:OE-BMP-7 plasmid injection in diabetic mice. b Input:whole protein lysis from the NRK-52E cells;IgG:the negative control group of IP was treated with IgG antibody;NG:normal-glucose control group;HG:high-glucose control group;HG + BMP-7:high glucose+BMP-7(200 ng/mL rhBMP-7);HG + MG132:high glucose+MG132(5 μmol/L). c Input:whole protein lysis from the NRK-52E cells;IgG:the negative control group of IP was treated with IgG antibody;NG:normal-glucose control group;HG:high-glucose control group;HG + BMP-7:high glucose+BMP-7(200 ng/mL rhBMP-7). d Schematic image of the molecular mechanisms involved in the role of BMP-7 in restoring SnoN protein level.BMP-7 upregulates SnoN mRNA and protein levels by activating the classical Smad1/5 pathway to refrain from the partial EMT of RTECs and the deposition of ECM in DKD-induced renal fibrosis.

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