Fig. 2: TFG deficiency downregulates autophagy-associated kinase ULK1 and increases p62 protein level. | Cell Death & Disease

Fig. 2: TFG deficiency downregulates autophagy-associated kinase ULK1 and increases p62 protein level.

From: TRK-fused gene (TFG) regulates ULK1 stability via TRAF3-mediated ubiquitination and protects macrophages from LPS-induced pyroptosis

Fig. 2

A THP-1 and U937 cells were transduced with shNC, shTFG#1, or shTFG#2. IB were performed to analyze ULK1 and p62 expression levels. B Relative ULK1 and p62 protein levels are shown as bar plots. Bars represent the mean ± s.d. *p < 0.05; n = 3 for each group. C THP-1 cells transduced with shNC or shTFG#2 were treated with CHX (20 μg/mL) for indicated times and IB assays were performed to analyze ULK1 degradation. D TFG-knockdown THP-1 cells (shTFG#2 transduced) were pretreated with lysosome inhibitor E64D (10 μg/mL) + pepstatin A (1 μg/mL) (E + P) or proteasome inhibitor MG132 (10 μg/mL) for 2 h. Cells were then treated with CHX (20 μg/mL) for indicated times and IB assay were performed to analyze ULK1 degradation. E Relative protein levels of ULK1 are shown. Data are shown as mean ± s.d.; n = 3 for each group. Experiments were independently repeated three times.

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