Fig. 7: Rescue of ULK1 expression blocks LPS/Ng-induced cell death in TFG-deficient THP-1 cells.

A THP-1 cells were transduced with shNC or shTFG. TFG stable knockdown THP-1 cells were then transfected with V5-TFG rescue or V5-TFG Δ11–91 rescue expression vectors to rescue TFG (shTFG + V5-TFGres) or TFG Δ11–91 expression (shTFG + V5-TFGΔ11-91res). Cells were stimulated with LPS/Ng and analyzed by SYTOX Red dead cell staining. B Scatter dot plots of cell death percentage in groups described in A are shown. Data are shown as mean ± s.d. *p < 0.05; n.s. no significance; n = 3 for each group. C THP-1 cells were transduced with shNC or shTFG. TFG stable knockdown THP-1 cells were then transfected with LV8N-(mcherry)-ULK1 to rescue ULK1 expression (shTFG + ULK1res). Cells were then treated with LPS/Ng and whole-cell lysates and supernatants were subjected to IB. D THP-1 cells were stimulated with LPS/Ng. Cell death were analyzed by SYTOX Red dead cell staining. E Scatter dot plots of cell death percentage in groups described in D are shown. Data are shown as mean ± s.d. *p < 0.05; n = 3 for each group. Experiments were independently repeated three times.