Fig. 4: Upregulation of LINC01123 or B7–H3 or downregulation of miR-214-3p induces dysfunction of CD8+T cells. | Cell Death & Disease

Fig. 4: Upregulation of LINC01123 or B7–H3 or downregulation of miR-214-3p induces dysfunction of CD8+T cells.

From: LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7–H3 in head and neck squamous-cell carcinoma

Fig. 4

CD8+T cells were isolated from PBMCs using immunomagnetic beads. After activation and amplification, CD8+T cells were cocultured with different CAL-27 cell treatment groups. A Expression of TNF-α, IFN-γ, perforin, and granzyme B in CD8+T-cell population was analyzed with flow cytometry. B Statistical analysis of the results from flow cytometry of CD8+T cells. C Expression levels of TNF-α, IFN-γ, perforin, and granzyme B in CAL-27–CD8+T cocultured supernatants, as detected by ELISA. D CCK8 cytotoxicity test of CD8+T cells. E ELISA was used to assess the immune activity of CD8+T cells after recombinant human B7–H3 treatment. *P < 0.05 compared with cells without treatment. The experiment was repeated three times.

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