Fig. 1: ANKHD1 knockdown enhanced the radiosensitivity of CRC both in vitro and in vivo. | Cell Death & Disease

Fig. 1: ANKHD1 knockdown enhanced the radiosensitivity of CRC both in vitro and in vivo.

From: The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling

Fig. 1

A ANKHD1 expression levels were tested by Western blot in HCT116 and HCT8 cells infected with lentivirus-targeting ANKHD1, the silencing efficiency was analyzed using Image J (*P < 0.05, **P < 0.01). B ANKHD1 silencing increased the radiosensitivity of HCT116 and HCT8 colorectal cancer cells (SER = 1.378 in HCT116, SER = 1.225 in HCT8). C The D0, N, Dq, and SER values of ANKHD1-silenced cells, the SER value was simulated using the multi-target single-hit model. D, E Tumor-growth curves and images of all tumors of each group (n = 5 and 4 tumors were observed). F The quality of each group tumors (*P < 0.05, **P < 0.01). G H&E staining of tumor section of all groups in this study, IHC was performed to test the expression of ANKHD1 and γH2AX in the tumor section of all groups in this study. H The expression of ANKHD1 in subcutaneous tumor tissues was detected by Western blot.

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