Fig. 3: MALAT1 knockdown enhanced radiosensitivity by inhibiting DDR in CRC cells. | Cell Death & Disease

Fig. 3: MALAT1 knockdown enhanced radiosensitivity by inhibiting DDR in CRC cells.

From: The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling

Fig. 3

A MALAT1 expression level was tested by qRT-PCR in HCT116 cells infected with lentivirus-targeting MALAT1 (**P < 0.01). B MALAT1 silencing decreased the survival fraction of HCT116 cells post IR, SER = 1.379. C The D0, N, Dq and SER values of MALAT1-silenced cells, the SER value was simulated using the multitarget single-hit model. D γH2AX foci were detected by immunofluorescence at 0, 0.5, 4, and 12 h post IR in CHT116 cell, and the number of γH2AX foci was counted in more than 100 cells (**P < 0.01). E The expression of γH2AX was tested at 0, 0.5, 4 and 12 h after 4-Gy IR. F The formation of ROS was tested by flow cytometry at 0.5 h after 4 Gy IR in MALAT1-silenced HCT116 cells (*P < 0.05, ***P < 0.001). G The expression of p-ATM, ANKHD1, 53BP1, RAD50, MRE11, YAP1, p-CHK2, and CHK2 was detected by Western blotting at 0, 0.5, and 4 h after 4 Gy IR in MALAT1-silenced cells.

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