Fig. 4: The interaction between ANKHD1 and YAP1 stabilizes the transcriptional activity of YAP1. | Cell Death & Disease

Fig. 4: The interaction between ANKHD1 and YAP1 stabilizes the transcriptional activity of YAP1.

From: The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling

Fig. 4

A The expression of YAP1 was tested by IHC in colorectal cancer tissue and normal tissue, and the staining of YAP1 in tumors was divided into three levels: low staining, moderate staining, and high staining. B The chi-square test showed an obvious difference in YAP1 expression between colorectal tumors and normal tissue (top, P < 0.005), and the chi-square test showed a high correlation of the expression of ANKHD1 and YAP1 in colorectal tumors (bottom, P < 0.05). C The correlation between the expression of ANKHD1 and YAP1 was analyzed in CRC using the GEPIA database (P < 0.05). D Immunoprecipitation indicated that ANKHD1 could bind to YAP1 in HCT116 cells. E A nucleus-cytoplasm separation assay showed that ANKHD1 silencing decreased YAP1 expression in the nucleus and increased the phosphorylation of YAP1 in the cytoplasm. F The location of YAP1 was measured by immunofluorescence, indicating that ANKHD1 silencing inhibited the nuclear translocation of YAP1. G qRT-PCR was performed to detect the mRNA expression of ANKHD1, YAP1, and CTGF, and the data are presented as the mean ± SD (*P < 0.05, ***P < 0.001). H Western blot analysis showed that ANKHD1 silencing increased the degradation of ANKHD1 and YAP1 after CHX treatment (10 μg/ml).

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