Fig. 6: YAP1 is an important regulator of the DDR.

A Western blotting was used to detect the expression of YAP1 in HCT116 cells transfected with overexpression plasmid or siRNA targeting YAP1. B The expression of γH2AX was detected by Western blotting at 0, 0.5, 4, and 12 h after 4 Gy IR in HCT116 cells with upregulated or downregulated expression of YAP1. C γH2AX foci were detected by immunofluorescence at 0.5 h post IR in HCT116 cells with upregulated or downregulated expression of YAP1, and the number of γH2AX foci was counted in more than 100 cells (**P < 0.01). D YAP1 expression affected the formation of ROS in HCT116 cells (*P < 0.01). E Downregulation of YAP1 induced cell arrest in G2/M phase at 12 h post IR (***P < 0.001). F The expression of p-ATM, RAD50, MRE11, NBS1, YAP1, p-CHK2, and CHK2 was tested by Western blot at 0, 0.5, and 4 h post IR in HCT116 cells with upregulated or downregulated expression of YAP1. G Downregulation of YAP1 increased apoptosis at 48 h post IR, and the data are presented as the mean ± SD (*P < 0.05, **P < 0.01). H Downregulation of YAP1 increased the expression of cleaved-caspase 3 at 48 h post IR.