Fig. 3: ALCAM suppresses the hedgehog pathway activation.
From: ALCAM regulates multiple myeloma chemoresistant side population
A GSEA analysis showing the hedgehog pathway gene enrichment in SP and MP cells; B Heatmap showing differentially regulated hedgehog pathway gene expression. From left to right are comparisons of CK-KD SP vs. AL-KD SP, CK-KD MP vs. AL-KD MP, CK-KD MP vs. CK-KD SP, and AL-KD MP vs. AL-KD SP. p < 0.05 for all listed genes. C Reverse transcriptional quantitative PCR for hedgehog pathway gene expression in CTR-KD versus AL-KD MM cells. D Hoechst staining of CTR-KD or AL-KD RPMI8226 cells, cocultured with BMSC in the presence of GLI inhibitor (GNAT61, 10 μΜ) and the result quantification (below panel). E Hoechst staining of CTR-KD or AL-KD RPMI8226 cells, cocultured with BMSC or recombinant EGF protein (10 ng/ml) in the presence of SMO inhibitor (cyclopamine, 50 nM) for 24 h and the result quantification (right panel). Data are the mean of three independent experiments in three replicates. *p < 0.05.
