Fig. 2: Downregulation of HDAC1 represses the growth of CC cells in vivo and in vitro. | Cell Death & Disease

Fig. 2: Downregulation of HDAC1 represses the growth of CC cells in vivo and in vitro.

From: Suppression of histone deacetylase 1 by JSL-1 attenuates the progression and metastasis of cholangiocarcinoma via the TPX2/Snail axis

Fig. 2

A Knockdown efficiency of shHDAC1#1 and shHDAC1#2 in TFK-1 cells detected by Western blot analysis. B Proliferation of TFK-1 cells upon HDAC1 silencing assessed by trypan blue staining. C The number of newly formed colonies in TFK-1 cells upon HDAC1 silencing tested by colony formation assay. D Tumor volume in nude mice in response to HDAC1 silencing. E Tumor weight in nude mice in response to HDAC1 silencing. F Immunohistochemical analysis of Ki67 and HDAC1 proteins in the tumor tissues of nude mice in response to HDAC1 silencing. G HDAC1 protein expression in tumor tissues of nude mice in response to HDAC1 silencing measured by Western blot analysis. *p < 0.05. In panels (A) and (C), data were compared by one-way ANOVA with Tukey’s post hoc test. In panels (E–G), data were compared by unpaired t-test. In panels (B) and (D), data were compared by repeated measures ANOVA with Bonferroni post hoc test. The cell experiment was independently repeated three times. n = 6 for animal experiment.

Back to article page