Fig. 4: HDAC1 loss-of-function represses the growth of CC cells via suppression of TPX2. | Cell Death & Disease

Fig. 4: HDAC1 loss-of-function represses the growth of CC cells via suppression of TPX2.

From: Suppression of histone deacetylase 1 by JSL-1 attenuates the progression and metastasis of cholangiocarcinoma via the TPX2/Snail axis

Fig. 4

A TPX2 expression upon HDAC1 silencing determined by RT-qPCR. B TPX2 protein expression in TFK-1 cells upon HDAC1 silencing measured by Western blot analysis. C The transfection efficiency of sh-HDAC1 and oe-TPX2 in TFK-1 cells tested by Western blot analysis. D Proliferation of TFK-1 cells shown by trypan blue staining after HDAC1 silencing alone or with TPX2 overexpression in combination. E The number of newly formed colonies in TFK-1 cells tested by colony formation assay after HDAC1 silencing alone or with TPX2 overexpression in combination. F, G Quantitative analysis of xenografted tumor volume (F) and weight (G) after HDAC1 silencing alone or with TPX2 overexpression in combination. H Immunohistochemical detection of Ki67, TPX2 and HDAC1 proteins in tumor tissues of nude mice after HDAC1 silencing alone or with TPX2 overexpression in combination. I, TPX2 mRNA expression in TFK-1 cells overexpressing HDAC1 measured by RT-qPCR. J, TPX2 and HDAC1 protein expression in TFK-1 cells overexpressing HDAC1 or combined with TPX2 silencing measured by Western blot analysis. *p < 0.05 versus the Sceamble group. # p < 0.05 versus the shHDAC1 or the shHDAC1 + NC group. In panels (A–D), (F–H) and (J), data were compared by one-way ANOVA with Tukey’s post hoc test. In panels (E) and (I), data were compared by repeated measures ANOVA with Bonferroni post hoc test. The cell experiment was independently repeated three times. n = 6 for animal experiment.

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