Fig. 7: JSL-1 impedes the proliferation of CC cells and tumorigenesis via repression of Snail/TPX2. | Cell Death & Disease

Fig. 7: JSL-1 impedes the proliferation of CC cells and tumorigenesis via repression of Snail/TPX2.

From: Suppression of histone deacetylase 1 by JSL-1 attenuates the progression and metastasis of cholangiocarcinoma via the TPX2/Snail axis

Fig. 7

A Acetylation level of H3K9 and H4K16 and expression of Snail and TPX2 proteins in TFK-1 cells upon JSL-1 treatment. B Viability of TFK-1 cells upon JSL-1 treatment at different doses detected by MTT assay. C Number of colonies in TFK-1 cells following JSL-1 treatment assessed by colony formation assay. D Tumor volume of nude mice upon JSL-1 treatment. E Tumor weight of nude mice upon JSL-1 treatment. F Ki67-positive cells and positive Snail and TPX2 protein expression in tumor tissues of nude mice treated with JSL-1 detected by immunohistochemistry. G The expression of Snail and TPX2 proteins and acetylation level of H3K9 and H4K16 in tumor tissues of nude mice after JSL-1 treatment measured by Western blot analysis. *p < 0.05. In panel (A), data were compared by one-way ANOVA with Tukey’s post hoc test. In panels (E–G), data were compared using unpaired t-test. In panels (B–D), data were compared using repeated measures ANOVA with Bonferroni post hoc test. The cell experiment was independently repeated three times. n = 6 for animal experiment.

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