Fig. 3: Effect of ectopic expression of cFLIP on ER stress-induced caspase-8 activation and apoptosis. | Cell Death & Disease

Fig. 3: Effect of ectopic expression of cFLIP on ER stress-induced caspase-8 activation and apoptosis.

From: cFLIP downregulation is an early event required for endoplasmic reticulum stress-induced apoptosis in tumor cells

Fig. 3

HCT116 pBABE-ø, pBABE-cFLIPL, pLPCX-ø, and pLPCX-cFLIPS cells were treated or not with TG (100 nM). A Apoptosis was analyzed after 30 h treatment by subG1 analysis as described under Materials and Methods (****p ≤ 0.0001; two-way ANOVA. Tukey’s multiple comparisons test). B cFLIP levels and caspase-8 processing were determined in whole-cell extracts by western blotting after 24-h treatment. GAPDH was used as a protein loading control. Blots are representative of three independent experiments.

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