Fig. 4: Effect of cFLIP knockdown on ER stress-induced caspase-8 activation and apoptosis. | Cell Death & Disease

Fig. 4: Effect of cFLIP knockdown on ER stress-induced caspase-8 activation and apoptosis.

From: cFLIP downregulation is an early event required for endoplasmic reticulum stress-induced apoptosis in tumor cells

Fig. 4

A HCT116 cells were transfected with scrambled oligonucleotide (Sc) or FLIPL, FLIPS, or FLIPDUAL siRNAs for 48 h prior to TG (100 nM) treatment for the indicated times and apoptosis was determined by subG1 analysis. cFLIP knockdown was assessed in whole-cell extracts by western blotting. GAPDH was used as protein loading control. B HCT116 pBABE-ø or pBABE-cFLIPL cells were transfected for 48 h with scrambled oligonucleotide (Sc) or FLIPL siRNAs and apoptosis was determined after a further 24 h of thapsigargin treatment (ns = not statistically significant; ****p ≤ 0.0001; two-way ANOVA. Tukey’s multiple comparisons test). cFLIPL levels were assessed by western blotting. C HCT116 cells were either non-transfected (−) or transfected with scrambled oligonucleotide (Sc) or cFLIPL siRNA for 48 h prior to TG treatment for the indicated times. cFLIP protein levels and caspase-8 processing were determined in whole-cell extracts by western blotting. GAPDH levels were used as protein loading control. Blots are representative of three independent experiments.

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