Fig. 1: Fascin is highly expressed in TNBC and enhances cell metastasis.
From: Fascin enhances the vulnerability of breast cancer to erastin-induced ferroptosis
A Fascin expression statuses (unit: log2(norm_count+1)) in TNBC compared to non-TNBC samples were obtained from the TCGA database. B Fascin expression statuses (unit: log2 gene expression data) in TNBC and non-TNBC samples were obtained from GSE76275. C All examined breast cancer cell lines were subjected to qRT-PCR to measure Fascin mRNA levels. D All examined breast cancer cell lines were subjected to Western blot analysis to measure Fascin protein levels. E IHC analysis of Fascin level in TNBC. F Wound‐healing assays were conducted in HS578T and MDAMB231 cells transfected with siCon and siFascin. Migration distance was measured at 0 and 24 h after cells were scratched. G Transwell migration and matrigel invasion assays were conducted in HS578T and MDAMB231 cells transfected with siCon and siFascin. The cells that adhered to the membrane were imaged and counted. H Induction of EMT-related proteins upon Fascin knockdown in MDAMB231 cells. Cell lysates were collected after 72 h transfection. I Morphological changes of MDAMB231 cells transfected with siCon and siFascin. J The Kaplan–Meier Plotter database (https://kmplot.com/analysis/) was used to show the prognostic value of Fascin in breast cancer patients. HR, hazard ratio. **p < 0.01, ***p < 0.001. The data are displayed as the means ± s.d of three independent experiments.
