Fig. 3: Fascin silencing inhibits ferroptosis sensitivity in the HS578T and MDAMB231 cell lines.
From: Fascin enhances the vulnerability of breast cancer to erastin-induced ferroptosis
The effects of Fascin knockdown on mRNA (A) and protein (B) levels compared to negative controls. HS578T (C) and MDAMB231 (D) cell lines transfected with Fascin siRNAs or siCon were incubated with the indicated concentrations of erastin for 48 h and then assayed for cell viability. E, F MDAMB231 cell lines transfected with Fascin siRNAs or siCon were incubated with erastin (7.5 μM) for 48 h and then assayed to flow cytometry analysis for cell live/dead double staining. HS578T (G) and MDAMB231 (H) cell lines transfected with Fascin siRNAs or siCon were incubated with erastin (5 μM) for 24 h, stained with C11-BODIPY, and then subjected to flow cytometry analysis. HS578T (I) and MDAMB231 (J) cell lines transfected with Fascin siRNAs or siCon were incubated with erastin (0.25 μM) for 48 h and then assayed for GSH levels. K MDAMB231 cells transfected with Fascin siRNAs or siCon were incubated with erastin (5 μM) for 24 h and then used for TEM analysis. The white arrows show dysmorphic mitochondria (smaller mitochondria, condensed membrane, and decreased cristae). n.s. not significant. *p < 0.05, **p < 0.01, and ***p < 0.001. The data are displayed as the means ± s.d of three independent experiments.
