Fig. 4: Fascin overexpression promotes ferroptosis sensitivity of MDAMB231 cell lines.
From: Fascin enhances the vulnerability of breast cancer to erastin-induced ferroptosis
The effects of Fascin overexpression on mRNA (A) and protein (B) expression levels compared to the corresponding empty vectors. C MDAMB231 cells transfected with an expression vector for Fascin or the corresponding empty vectors were incubated with the indicated concentrations of erastin for 48 h and then assayed for cell viability. D, E MDAMB231 cells transfected with an expression vector for Fascin or the corresponding empty vectors were incubated with erastin (5 μM) for 48 h and then assayed to flow cytometry analysis for cell live/dead double staining. F MDAMB231 cells transfected with an expression vector for Fascin or the corresponding empty vectors were incubated with erastin (2.5 μM) for 24 h, stained with C11-BODIPY, and then subjected to flow cytometry analysis. G MDAMB231 cells transfected with an expression vector for Fascin or the corresponding empty vectors were incubated with erastin (0.25 μM) for 48 h and then assayed for GSH levels. n.s. not significant. *p < 0.05, and ***p < 0.001. The data are displayed as the means ± s.d of three independent experiments.
