Fig. 7: Ferroptosis induction as a novel treatment to suppress tamoxifen-resistant cells.
From: Fascin enhances the vulnerability of breast cancer to erastin-induced ferroptosis
A Relative Fascin status in wild-type MCF7 and tamoxifen-resistant MCF7/TAMR cells. B–E A total of 664 differentially expressed proteins were analyzed for GO annotations and KEGG enrichments. A total of 255 upregulated proteins were analyzed for GO annotations (B) and KEGG enrichments (D). A total of 409 downregulated proteins were analyzed for GO annotations (C) and KEGG enrichments (E). F MCF7 and MCF7/TAMR cells were incubated with the indicated concentrations of erastin for 48 h and then assayed for cell viability. G Western blot analysis showing that Fascin knockdown increases xCT levels in MCF7/TAMR cells. Cell lysates were collected after 72 h transfection. H MCF7/TAMR cells transfected with Fascin siRNAs or siCon were incubated with the indicated concentrations of erastin for 48 h and then assayed for cell viability. I MCF7/TAMR cells were incubated for 48 h with or without 20 or 30 μM 4-OH tamoxifen and in the absence or presence of 5 μM erastin. n.s. not significant. **p < 0.01, and ***p < 0.001. The data are displayed as the means ± s.d of three independent experiments.
