Fig. 2: Expression of inflammatory mediators decreases in the presence of the mitoNEET inhibitor NL-1.
From: Inhibition of mitoNEET attenuates LPS-induced inflammation and oxidative stress
Total RNA, cell supernatants, and total protein were harvested from RAW264.7 cells 12 h (for mRNA) and 24 h (for protein) after treatment with vehicle, NL-1 (20 µM), LPS (100 ng/mL), or LPS plus NL-1 (20 µM). Levels of mRNA encoding pro-inflammatory mediators IL-1β, IL-6, TNFα, iNOS, and COX2 were measured by quantitative real-time RT-PCR (A–E). Protein levels of pro-inflammatory mediators were analyzed using ELISA or western blotting (F–I). All data are presented as the mean ± SD from three independent experiments. *P < 0.05 for LPS vs. LPS plus NL-1.
