Fig. 2: ROCK2 suppression induced DSB in TMZ-R cells. | Cell Death & Disease

Fig. 2: ROCK2 suppression induced DSB in TMZ-R cells.

From: Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2

Fig. 2

A ROCK2 protein expression in TMZ-R cells and parental cells. B, C ROCK2 protein level was determined with ROCK2-shRNA (ROCK2-KD). D WB quantification of B and C. E Neutral comet assay was performed, Scale bars, 60 μm. F–I DSB-ChIP quantification over time of γH2AX level at the site-specific DSB. J γH2AX protein level in ROCK2-KD cells with TMZ (100 μM) for 24 h. K IF results of γH2AX-foci in U87R and U251R with ROCK2-KD. Data are mean ± SD of three independent experiments. p values determined by ANOVA or two-tailed unpaired t-test. Statistical differences compared with the controls are given as *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.

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