Fig. 3: ROCK2-KD suppressed stepwise recruitment of HR factors to DNA DSBs. | Cell Death & Disease

Fig. 3: ROCK2-KD suppressed stepwise recruitment of HR factors to DNA DSBs.

From: Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2

Fig. 3

A, B ROCK2-KD suppressed HR repair. C–F Heat maps of relative occupancy of indicated factors at site-directed DSB with ROCK2-KD. G Immunofluorescence images of RAD51 in cell nuclei in the indicated cells with TMZ (100 μM), Scale bars, 20 μm. H Quantification of RAD51 foci-positive nuclei. I Schematic representation of PCR-based DNA end-resection assay. J Quantification of end-resection at the same site-specific DSB as used in the DSB-ChIP assay. Data are expressed as mean ± SD. p values determined by ANOVA or two-tailed unpaired t-test. Statistical differences compared with the controls are given as *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.

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