Fig. 4: Enhancement of NHEJ by ROCK2 inhibition was useless for DNA repair in TMZ-R cells. | Cell Death & Disease

Fig. 4: Enhancement of NHEJ by ROCK2 inhibition was useless for DNA repair in TMZ-R cells.

From: Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2

Fig. 4

A–D NHEJ versus time was determined in ROCK2-KD cells. E–G γH2AX foci-positive cells were detected by IF and counted, Scale bars, 20 μm. H Ku80 expression was determined with Ku80-overexpression plasmids. I–K NHEJ for 24 h was determined in ROCK2-KD cells with Ku80-OE. L–N Cells were counted with TMZ and Ku80-OE in ROCK2-KD cells. O Cells were stained with TMZ and Ku80-OE. Data are shown as mean ± SD of three independent experiments. p values determined by ANOVA t-test. Statistical differences compared with the controls are given as *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.

Back to article page