Fig. 7: ROCK2 mediated ATM expression via ZEB1. | Cell Death & Disease

Fig. 7: ROCK2 mediated ATM expression via ZEB1.

From: Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2

Fig. 7

A Different wild-type ATM promoter luciferase reporter constructs were used for binding assay. B, C ATM gene expression of U87R and U251R was determined with different siRNA of TFs as indicated. D ATM gene expression was determined with ZEB1 knock-down. E–G ATM protein expression was determined with ZEB1 knock-down. WB results: E, Quantification of WB: F and G. H ATM gene expression was determined with ZEB1 overexpression. I–K ATM protein expression was determined with ZEB1 overexpression. WB results: I, Quantification of WB: J and K. L ATM gene expression was determined with ZEB1-tet-KD in ROCK2-OE cells. M–O ATM gene expression was determined with ZEB1-tet-KD in ROCK2-OE cells. WB results: M, Quantification of WB: N and O. Data are shown as of three independent experiments. p values determined by ANOVA or two-tailed unpaired t-test. Statistical differences compared with the controls are given as *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.

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