Fig. 1: Serum starvation induces autophagy and apoptosis in endothelial cells.
From: Autolysosomes and caspase-3 control the biogenesis and release of immunogenic apoptotic exosomes
A Evaluation by Hoechst 33342 and propidium iodide (HO/PI) staining of apoptosis and necrosis in HUVECs exposed to normal medium (N) or serum-starved (SS) for 4 h. P values obtained by unpaired t test. n = 10 for each condition. Scale bar = 25 μm. B Quantification of caspase-3 activity in HUVECs exposed to N or SS HUVECs for 1, 2, 3, and 4 h. P values obtained by one-way ANOVA. n = 4 for each condition. C Immunoblot and densitometric analysis of PARP, LC3, and SQSTM1/p62 in HUVECs exposed to N or SS for 1, 2, or 4 h. β-actin (ACTB) was used as a loading control. P values obtained by unpaired t test (*P < 0.05; **P < 0.01; ***P < 0.001). n = 3 for each condition. All values are expressed as the mean ± SEM.
