Fig. 1: Irg-1 expression in mice with spinal cord injury and LPS-stimulated microglia. | Cell Death & Disease

Fig. 1: Irg-1 expression in mice with spinal cord injury and LPS-stimulated microglia.

From: Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice

Fig. 1

A The data from the GEO DataSets (https://www.ncbi.nlm.nih.gov/gds), series accession: GDS2159/1427381. B Modeling mode of moderate spinal cord injury in mice. C The level of Irg-1 mRNA at 0.5, 4, and 24 h after spinal cord injury in mice were detected by qRT-PCR. D, E The Irg-1 protein levels at 0.5, 4, and 24 h after spinal cord injury in mice were detected by western blot and quantified by Image J software. F–I The inflammation level and Irg-1 protein expression of microglia under different time and concentration of LPS stimulation were detected by western blot. The data are presented as the mean ± SD. n = 5. NS not significant, *P < 0.05, **P < 0.01.

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