Fig. 2: The effect of knockdown and overexpression of Irg-1 on microglia stimulated by LPS. | Cell Death & Disease

Fig. 2: The effect of knockdown and overexpression of Irg-1 on microglia stimulated by LPS.

From: Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice

Fig. 2

A, B, G, H The efficiency of Irg-1 knockdown or overexpression was tested by western blot and quantified by Image J software. C, I The mRNA expression of iNOS, COX2 and IL-6 were measured by qRT-PCR in the microglia, treated with LV-shIrg-1 or LV-Irg-1. D, E, J, K The protein expression of Irg-1, iNOS, COX2 and IL-6 were detected by western blot. F, L The levels of PGE2, TNF-α, IL-6, IL-1β were detected by ELISA. The data are presented as the mean ± SD. n = 5. NS not significant, *P < 0.05, **P < 0.01.

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