Fig. 4: Effect of Irg-1 on inflammation and functional recovery in mice with spinal cord injury. | Cell Death & Disease

Fig. 4: Effect of Irg-1 on inflammation and functional recovery in mice with spinal cord injury.

From: Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice

Fig. 4

A, B The effect of lentivirus on spinal cord injury in mice was detected at day 14 by western blot and quantified by Image J software. C, D Basso mouse scale (BMS) and inclined plane test for the indicated groups at days 1, 3, 7, 14, 21, and 28 (n = 8–12). #P < 0.05, ##P < 0.01 relative to the SCI+LV-Ctrl group. E Photos of mice footprints at day 28 after SCI. F, G Quantification of stride width and toe dragging in the footprint analysis. H The transected spinal cord sections from each group were analyzed at day 28 by HE staining and Nissl staining. The black arrows indicate motor neurons (scale bar: 100 μm and 50 μm). I Quantitative analysis of Nissl positive motor neurons in the anterior horn of spinal cord in each group. J, K The iNOS/NeuN in spinal cord injury was detected at day 28 by immunofluorescence combined with DAPI staining for nuclei (scale bar: 25 μm). The data are presented as the mean ± SD. n = 6 (except for BMS and inclined plane test). *P < 0.05, **P < 0.01.

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