Fig. 5: 4-OI reduces LPS-induced inflammation in microglia. | Cell Death & Disease

Fig. 5: 4-OI reduces LPS-induced inflammation in microglia.

From: Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice

Fig. 5

A Chemical structure of 4-OI. B, C Cell Counting Kit-8 (CCK-8) results for microglia treated with different concentrations of 4-OI for 24 and 48 h. D–F The mRNA expression of iNOS, COX2 and IL-6 were measured by qRT-PCR. G, H The protein expression of iNOS, COX2 and IL-6 were measured by western blot and quantified by Image J software. I–L The expression of PGE2, TNF-α, IL-1β and IL-6 in microglia were measured through ELISA assay. The data are presented as the mean ± SD. n = 5. NS not significant, *P < 0.05, **P < 0.01.

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