Fig. 6: Effect of 4-OI on the polarization of microglia. | Cell Death & Disease

Fig. 6: Effect of 4-OI on the polarization of microglia.

From: Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice

Fig. 6

A, B The Iba-1 was detected by immunofluorescence combined with DAPI staining for nuclei (scale bar: 50 μm). C The morphology of microglia after adding 4-OI (scale bar: 100 μm). D, E The CD68/Arg-1 was detected by immunofluorescence combined with DAPI staining for nuclei (scale bar: 50 μm). F The mRNA levels of CD68 and Arg-1 were detected by qRT-PCR. G, H The protein expression of Iba-1, CD68, Arg-1 were detected by western blot and quantified by Image J software. The data are presented as the mean ± SD. n = 5. NS not significant, *P < 0.05, **P < 0.01.

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