Fig. 1: Deletion of epithelial Cd44 reduces Wnt signaling in murine intestinal crypts.
From: Wnt signaling is boosted during intestinal regeneration by a CD44-positive feedback loop
a Confocal image of IF staining on sections of the small intestine (SI) of Cd44Δie and Cd44+/+ mice using anti-lysozyme antibodies. Scale bar: 50 µm. b Quantification of lysozyme-stained PCs in the crypts of Cd44Δie and Cd44+/+ mice. Error bars, ±SE. c qPCR of lysozyme and α-defensin5 (Lyz and Defa5) expression in intestinal crypts from Cd44Δie and Cd44+/+ mice. Gapdh and β-Actin were used as reference genes. Error bars, ±SE. d Nuclear β-catenin (arrowheads) IHC staining. Scale bar: 25 μm. e In situ hybridization (ISH) (brown, red) of Lgr5 and Axin2 expression in the SI. Positive control: Ppib. Error bars, ± SE. Scale bar: 50 µm. f, g Quantification of ISH. Error bars, ±SE. h qPCR of Wnt target genes and stem cell marker expression in SI crypts isolated from Cd44Δie and Cd44+/+ mice. Non-Wnt-regulated control: Cxcr4. Error bars, ±SE. Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. N = number of mice, n = number of crypts.
