Fig. 2: Conserved numbers but impaired functionality of stem cells in Cd44Δie mice.
From: Wnt signaling is boosted during intestinal regeneration by a CD44-positive feedback loop
a IHC staining of OLFM4. Scale bar: 50 μm. b Average number of OLFM4-positive stem cells per crypt. Error bars, ±SE. N = number of mice, n = number of crypts. c SI organoids observed 5 days after Cd44 deletion and controls. Scale bar: 200 µm. d Budded vs. round organoid numbers after deletion of Cd44 and passaging (P) every 5 days. n = number of organoids. Error bars, ±SE. e Representative images of f. Scale bar: 200 µm. f Relative organoid number along passages in Cd44fl/fl;VillinCreERT2 organoid cultured with or without 4-OHT treatment. N = 3 mice. Scale bar: 500 µm. g Organoid vs. cystic organoid numbers after deletion of Cd44 and treatment with Wnt3a CM, control CM or CHIR. N = 3 experiments. Error bars, ±SE. h Representative images of g CHIR and Wnt3a conditions. Scale bar: 200 µm. Student’s t test; *p < 0.05, **p < 0.01, ns = not significant. 4-OHT 4-hydroxytamoxifen. The dotted line in g indicates that the CHIR and Wnt3a conditions are two independent but related experiments.
